Section 9.4 – Standard Operating Procedures Followed

  1. Thin Layer Chromatography
  2. Plasma Free fatty acid analysis
  3. Deproteinising of samples
  4. Made PBS
  5. Manual Nucleic acid extraction
  6. PCR setup
  7. Restriction enzyme digest
  8. Agarose gel electrophoresis
  9. Checking gel
  10. DNA “clean-up” for sequencing
  11. Sanger sequencing with a capillary gel electrophoresis