Section 9 – Methods and Practicals
6 posts
Thin Layer Chromatography Plasma Free fatty acid analysis Deproteinising of samples Made PBS Manual Nucleic acid extraction PCR setup Restriction enzyme digest Agarose gel electrophoresis Checking gel DNA “clean-up” for 50
METHOD Performed Observed Neither Basic spectrophotometry Yes Making solutions Yes Standard curve Yes pH measurement Yes Agarose electrophoresis Yes CZE 50
A practical experiment to illustrate the measurement of total protein in serum using the Bradford assay